The brothers have been charged for not complying with Alberta's public health orders and holding a gathering at their church earlier today.
Dramatic footage of the arrests was captured and uploaded to YouTube:
The order, reports CTV's Kathy Le, "imposes new restrictions on organizers of protests and demonstrations requiring compliance with public health orders including masking, physical distancing and attendance limits."
Calgary Police claim they "served the Pawlowski brothers the court order prior to the church service", and did not enter the church.
Despite the order, the brothers allegedly "did not social distance, wear masks or reduce the capacity inside and carried on with the event."
The Calgary church gained national attention after ordering police to leave their property earlier this year.
Police said: "Artur Pawlowski and Dawid Pawlowski have both been arrested and charged with organizing an illegal in-person gathering, including requesting, inciting or inviting others to attend an illegal public gathering, promoting and attending an illegal public gathering.
"It is important to understand that law enforcement recognizes people's desire to participate in faith-based gatherings as well as the right to protest. However, as we find ourselves in the midst of a global pandemic, we all must comply with public health orders in order to ensure everyone's safety and wellbeing."